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Glycoside Hydrolase Family 94
| Glycoside Hydrolase Family 94 | |
| Clan | none (similar to GH-L) |
| Mechanism | inverting |
| Active site residues | known |
| CAZy DB link | |
| http://www.cazy.org/fam/GH94.html | |
Substrate specificities
This family contains phosphorolytic enzymes (usually named using a combination of “the substrate” and “phosphorylase”) that cleave beta glycosidic bond. The substrate specificities found in GH94 are: cellobiose (Glc-β1,4-Glc) phosphorylase (EC 2.4.1.20), cellodextrin ((Glc-β1,4-)n-1Glc; n≥3) phosphorylase (EC 2.4.1.29), (N.N’-diacetyl)chitobiose (GlcNAc-β1,4;-GlcNAc) phosphorylase, and a domain phosphorolyzing protein-bound β-1,2-glucan accompanied by cyclic β1,2-glucan synthase(EC 2.4.1.-) belonging to GT84.
Phosphorylases
Kinetics and Mechanism
Catalytic Residues
Three-dimensional structures
Family Firsts
- First sterochemistry determination
- First catalytic nucleophile identification
- First general acid/base residue identification
- First 3-D structure
- Cite some reference here, with a short explanation [1].
References
- Hidaka M, Honda Y, Kitaoka M, Nirasawa S, Hayashi K, Wakagi T, Shoun H, and Fushinobu S. (2004). Chitobiose phosphorylase from Vibrio proteolyticus, a member of glycosyl transferase family 36, has a clan GH-L-like (alpha/alpha)(6) barrel fold. Structure. 2004;12(6):937-47. DOI:10.1016/j.str.2004.03.027 |