Carbohydrate Esterase Family 1

This page is currently under construction. This means that the Responsible Curator has deemed that the page's content is not quite up to CAZypedia's standards for full public consumption. All information should be considered to be under revision and may be subject to major changes.

• Author: ^^^Casper Wilkens^^^
• Responsible Curator:

---

Carbohydrate Esterase Family 1
Clan	GH-x
Mechanism	retaining/inverting
Active site residues	known/not known
CAZy DB link
https://www.cazy.org/CE1.html

Substrate specificities

Carbohydrate esterase family 1 (CE1) is one of the biggest and most diverse CE families including acetylxylan esterases (EC 3.1.1.72), feruloyl esterases (EC 3.1.1.73), cinnamoyl esterases (EC 3.1.1.-), carboxylesterases (EC 3.1.1.1), S-formylglutathione hydrolases (EC 3.1.2.12), diacylglycerol O-acyltransferases (EC 2.3.1.20), and thehalose 6-O-mycolyltransferases (EC 2.3.1.122) and others [1].

Kinetics and Mechanism

Content is to be added here. Mycolyltransferases transfer of the mycolyl group from α,α′-trehalose monomycolate to a second α,α′-trehalose monomycolate molecule forming α,α′-trehalose dimycolate [2] Ferulic acid esterases hydrolyze the ester bond linking the ferulic acid to the arabinose moiety, which decorate certain types of xylan.

Catalytic Residues

Content is to be added here.

Three-dimensional structures

CE1's are members of the α/β-hydrolase superfamily [3], which are comprised of central β-strands connected by α-helices [4].

Family Firsts

First characterized

Content is to be added here.

First mechanistic insight

The crystal structure of Mycobacterium tuberculosis H37Rv mycolyltransferase in complex with the covalently bound inhibitor, diethyl phosphate gave the first insight into the mechanism, which involved the highly conserved catalytic Ser-Glu-His triad [3].

First 3-D structure

Mycobacterium tuberculosis H37Rv mycolyltransferase crystal structure in 2000 [3].

References

Error fetching PMID 24270786:
Error fetching PMID 10655617:
Error fetching PMID 9162010:
Error fetching PMID 1409539: