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Glycoside Hydrolase Family 144
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- Author: ^^^Koichi Abe^^^
- Responsible Curator: ^^^Masahiro Nakajima^^^
| Glycoside Hydrolase Family GH144 | |
| Clan | GH-x |
| Mechanism | retaining/inverting |
| Active site residues | known/not known |
| CAZy DB link | |
| https://www.cazy.org/GH144.html | |
Substrate specificities
The founding member of glycoside hydrolase family 144 is endo-β-1,2-glucanase (EC3.2.1.71) from a soil bacterium Chitinophaga pinensis, which hydrolize β-1,2-linked glucan to produce a wide spectrum of corresponding oligosaccharides. This family also includes a sophorohydrolase (nonreducing end), which cleaves shorter β-1,2-glucan to release sophorose (Glc-β-1,2-Glc). From sequence analysis, endo-β-1,2-glucanase activity was major one.
Authors may get an idea of what to put in each field from Curator Approved Glycoside Hydrolase Families. (TIP: Right click with your mouse and open this link in a new browser window...)
In the meantime, please see these references for an essential introduction to the CAZy classification system: [1, 2].
Kinetics and Mechanism
Content is to be added here.
Catalytic Residues
Content is to be added here.
Three-dimensional structures
Content is to be added here.
Family Firsts
- First stereochemistry determination
- Content is to be added here.
- First catalytic nucleophile identification
- Content is to be added here.
- First general acid/base residue identification
- Content is to be added here.
- First 3-D structure
- Content is to be added here.
References
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Davies, G.J. and Sinnott, M.L. (2008) Sorting the diverse: the sequence-based classifications of carbohydrate-active enzymes. The Biochemist, vol. 30, no. 4., pp. 26-32. Download PDF version.
- Cantarel BL, Coutinho PM, Rancurel C, Bernard T, Lombard V, and Henrissat B. (2009). The Carbohydrate-Active EnZymes database (CAZy): an expert resource for Glycogenomics. Nucleic Acids Res. 2009;37(Database issue):D233-8. DOI:10.1093/nar/gkn663 |