Glycoside Hydrolase Family 97

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• Author: ^^^Tracey Gloster^^^
• Responsible Curator: ^^^Gideon Davies^^^

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Glycoside Hydrolase Family GH97
Clan	Not assigned
Mechanism	Retaining and Inverting
Active site residues	Inferred
CAZy DB link
http://www.cazy.org/fam/GH97.html

Substrate specificities

Family 97 enzymes hydrolyse alpha-linked substrates; the two enzymes from this family that have been characterised to date have alpha-glucosidase (EC 3.2.1.20) and alpha-galactosidase (EC 3.2.1.22) activity [1]. The alpha-glucosidase from Bacteroides thetaiotaomicron has been characterised in the most detail, and has been demonstrated to hydrolyse substrates ranging from maltose to maltoheptaose in length, and those containing a-1,6-, a-1,3- and a-1,2-, as well as a-1,4-, linkages [2, 3].

Kinetics and Mechanism

Family GH97 is unusual as it contains both retaining and inverting enzymes. Both mechanisms are strongly dependent on the presence of calcium, which coordinates with the C2-OH group.

Catalytic Residues

Content is to be added here.

Three-dimensional structures

Content is to be added here.

Family Firsts

First sterochemistry determination

Cite some reference here, with a short (1-2 sentence) explanation [4].

First catalytic nucleophile identification

Cite some reference here, with a short (1-2 sentence) explanation [5].

First general acid/base residue identification

Cite some reference here, with a short (1-2 sentence) explanation [6].

First 3-D structure

Cite some reference here, with a short (1-2 sentence) explanation [7].

References

1. Gloster TM, Turkenburg JP, Potts JR, Henrissat B, and Davies GJ. (2008). Divergence of catalytic mechanism within a glycosidase family provides insight into evolution of carbohydrate metabolism by human gut flora. Chem Biol. 2008;15(10):1058-67. DOI:10.1016/j.chembiol.2008.09.005 |
2. Smith KA and Salyers AA. (1991). Characterization of a neopullulanase and an alpha-glucosidase from Bacteroides thetaiotaomicron 95-1. J Bacteriol. 1991;173(9):2962-8. DOI:10.1128/jb.173.9.2962-2968.1991 |
3. Kitamura M, Okuyama M, Tanzawa F, Mori H, Kitago Y, Watanabe N, Kimura A, Tanaka I, and Yao M. (2008). Structural and functional analysis of a glycoside hydrolase family 97 enzyme from Bacteroides thetaiotaomicron. J Biol Chem. 2008;283(52):36328-37. DOI:10.1074/jbc.M806115200 |

[[Category:Glycoside Hydrolase Families|GHnnn]]