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Glycoside Hydrolase Family 46
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- Author: ^^^Ryszard Brzezinski^^^
- Responsible Curator: ^^^Ryszard Brzezinski^^^
| Glycoside Hydrolase Family GHnn | |
| Clan | GH-I |
| Mechanism | inverting |
| Active site residues | known |
| CAZy DB link | |
| http://www.cazy.org/fam/GH46.html | |
Substrate specificities
Glycoside hydrolases of family 46 are essentially endo-beta-1,4-chitosanases (EC 3.2.1.132) that hydrolyze various links in chitosan, a polymer of beta-1,4-linked D-glucosamine (GlcN) units with a variable content (mostly 0 - 35%) of N-acetyl-D-glucosamine (GlcNAc) [1, 2]. Among the four types of links occurring between these two kinds of subunits in chitosan, all the enzymes examined for their cleavage specificity recognized productively the GlcN-GlcN links. Furthermore, the chitosanase from Bacillus circulans MH-K1 recognized also GlcN-GlcNAc links [3], while the chitosanase from Streptomyces sp. N174 recognized the GlcNAc-GlcN links [4].
Kinetics and Mechanism
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Catalytic Residues
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Three-dimensional structures
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Family Firsts
- First sterochemistry determination
- Cite some reference here, with a short (1-2 sentence) explanation (citation).
- First catalytic nucleophile identification
- Cite some reference here, with a short (1-2 sentence) explanation (citation).
- First general acid/base residue identification
- Cite some reference here, with a short (1-2 sentence) explanation (citation).
- First 3-D structure
- Cite some reference here, with a short (1-2 sentence) explanation (citation).
References
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Yabuki, M., Uchiyama, A., Suzuki, K., Ando, A., Fujii, T. (1988) Purification and properties of chitosanase from Bacillus circulans MH-K1. Journal of General and Applied Microbiology 34:255-270.
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Boucher, I., Dupuy, A., Vidal, P., Neugebauer, W. A., Brzezinski, R. (1992) Purification and characterization of a chitosanase from Streptomyces N174. Applied Microbiology and Biotechnology 38:188-193.
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Mitsutomi, M., Ueda, M., Arai, M., Ando, A., Watanabe, T. (1996) Action patterns of microbial chitinases and chitosanases on partially N-acetylated chitosan. Chitin Enzymology, vol. 2, pp 273-284.
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Fukamizo, T., Honda, Y., Goto, S., Boucher, I., Brzezinski, R. (1995) Reaction mechanism of chitosanase from Streptomyces sp. N174. Biochemical Journal 311:377-383.