Glycoside Hydrolase Family 44

This page is currently under construction. This means that the Responsible Curator has deemed that the page's content is not quite up to CAZypedia's standards for full public consumption. All information should be considered to be under revision and may be subject to major changes.

• Author: ^^^Peter Reilly^^^
• Responsible Curator: ^^^Peter Reilly^^^

---

Glycoside Hydrolase Family GH44
Clan	None specified
Mechanism	Retaining
Active site residues
CAZy DB link
https://www.cazy.org/GH44.html

Substrate specificities

Content is to be added here.

This is an example of how to make references to a journal article [1]. (See the References section below). Multiple references can go in the same place like this [1, 2]. You can even cite books using just the ISBN [3]. References that are not in PubMed can be typed in by hand [4].

Kinetics and Mechanism

Content is to be added here.

Catalytic Residues

Clostridium thermocellum: catalytic proton donor/acceptor: Glu186; catalytic nucleophile: Glu359. Clostridium acetobutylicum: catalytic proton donor/acceptor: Glu180; catalytic nucleophile: Glu352

Three-dimensional structures

Content is to be added here.

Family Firsts

First stereochemistry determination

Cite some reference here, with a short (1-2 sentence) explanation [1].

First catalytic nucleophile identification

Cite some reference here, with a short (1-2 sentence) explanation [4].

First general acid/base residue identification

Cite some reference here, with a short (1-2 sentence) explanation [2].

First 3-D structure

Cite some reference here, with a short (1-2 sentence) explanation [3].

References

2. He S and Withers SG. (1997). Assignment of sweet almond beta-glucosidase as a family 1 glycosidase and identification of its active site nucleophile. J Biol Chem. 1997;272(40):24864-7. DOI:10.1074/jbc.272.40.24864 |

4. Sinnott, M.L. (1990) Catalytic mechanisms of enzymic glycosyl transfer. Chem. Rev. 90, 1171-1202.

   DOI: 10.1021/cr00105a006

5. Kitago, Y., S. Karita, N. Watanabe, M. Kamiya, T. Aizawa, K. Sakka, and I. Tanaka. 2007. Crystal structure of Cel44A, a glycoside hydrolase family 44 endoglucanase from Clostridium thermocellum. J. Biol. Chem. 282, 35703–35711. DOI 10.1074/jbc.M706835200.

6. Nam, K. H., S.-J. Kim, and K. Y. Hwang. 2009. Crystal structure of CelM2, a bifunction­al glucanase–xylanase protein from a metagenomic library. Biochem. Biophys. Res. Comm. 383, 183–186.