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User:Hiroyuki Nakai
Hiroyuki Nakai is an assistant professor at Food Glycoscience Laboratory in Graduate School of Science and Technology, Niigata University. He obtained Ph. D from Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University in 2005, under the supervision of Prof. Atsuo Kimura and Prof. Seiya Chiba. He joined the group of Prof. Birte Svensson at Enzyme and Protein Chemistry, Department of Systems Biology, the Technical University of Denmark as post-doctoral fellow (2007) and assistant professor (2008-2010), working on chemoenzymatic synthesis of oligosaccharides by glycoside hydrolase and phosphorylase catalysing transglycosylation and reverse phosphorolysis, respectively. His recent research interests concern structure and function of Carbohydrate Active enZymes, enzymatic synthesis of oligosaccharides showing healthful effect on human intestine, antibacterial activity, and immunostimulatory activity.
- Nakai H., Abou Hachem M., Petersen B.O., Westphal Y., Mannerstedt K., Baumann M.J., Dilokpimol A., Schols H.A., Duus J.Ø., Svensson B. Efficient chemoenzymatic oligosaccharide synthesis by reverse phosphorolysis using cellobiose phosphorylase and cellodextrin phosphorylase from Clostridium thermocellum. Biochimie (2010) 92, 1818–1826.
- Nakai H., Baumann M.J., Petersen B.O., Westphal Y., Abou Hachem M., Dilokpimol A., Duus J.Ø., Schols H.A., Svensson B. Aspergillus nidulans α-galactosidase of glycoside hydrolase family 36 catalyses the formation of α-galacto-oligosaccharides by transglycosylation. The FEBS Journal (2010) 277, 3538–3551.
- Nakai H., Petersen B.O., Westphal Y., Dilokpimol A., Abou Hachem M., Duus J.Ø., Schols H.A., Svensson B. Rational engineering of Lactobacillus acidophilus NCFM maltose phosphorylase into either trehalose or kojibiose dual specificity phosphorylase. Protein Engineering Design & Selection (2010) 23, 781–787.
- Nakai H., Dilokpimol A., Abou Hachem M., Svensson B. Efficient one-pot enzymatic synthesis of α-(1→4)-glucosidic disaccharides through a coupled reaction catalysed by Lactobacillus acidophilus NCFM maltose phosphorylase. Carbohydrate Research (2010) 345, 1061–1064.
- Nakai H., Baumann M.J., Petersen B.O., Westphal Y., Schols H.A., Dilokpimol A., Abou Hachem M., Lahtinen S.J., Duus J.Ø., Svensson B. The maltodextrin transport system and metabolism in Lactobacillus acidophilus NCFM and production of novel α-glucosides through reverse phosphorolysis by maltose phosphorylase. The FEBS Journal (2009) 276, 7353–7365.