Glycoside Hydrolase Family 121

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• Author: ^^^Kiyotaka Fujita^^^
• Responsible Curator: ^^^Shinya Fushinobu^^^

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Glycoside Hydrolase Family GH121
Clan	GH-x
Mechanism	retaining
Active site residues	not known
CAZy DB link
https://www.cazy.org/GH121.html

Substrate specificities

This family of glycoside hydrolases contains β-L-arabinobiosidase, which was recently established for HypBA2 from Bifidobacterium longum JCM 1217[1].

Kinetics and Mechanism

HypBA2 is a retaining enzyme.The stereochemical course of the reaction was shown by transglycosylation activity toward 1-alkanols, and following ¹H-NMR and ¹³C-NMR analysis of the methanol-adducted transglycosylation product.

Catalytic Residues

Not known.

Three-dimensional structures

Not known.

Family Firsts

First stereochemistry determination

This was determined with HypBA2 enzyme by measurement of glycosyl transfer reactions to methanol and the ¹H-NMR and ¹³C-NMR spectra.

First catalytic nucleophile identification

No experimental proof.

First general acid/base residue identification

No experimental proof.

First 3-D structure

Not known.

References

1. Fujita K, Sakamoto S, Ono Y, Wakao M, Suda Y, Kitahara K, and Suganuma T. (2011). Molecular cloning and characterization of a beta-L-Arabinobiosidase in Bifidobacterium longum that belongs to a novel glycoside hydrolase family. J Biol Chem. 2011;286(7):5143-50. DOI:10.1074/jbc.M110.190512 |