Polysaccharide Lyase Family 8

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• Author: ^^^Michael Suits^^^
• Responsible Curator: ^^^Michael Suits^^^

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Polysaccharide Lyase Family PL8
3D Structure	(α/α)6 barrel + anti-parallel β-sheet
Mechanism	β-elimination
Active site residues	known/not known
CAZy DB link
https://www.cazy.org/PL8.html

Known Activities

PL8s display a broad range of enzymatic activities including: hyaluronate lyase (EC 4.2.2.1); chondroitin AC lyase (EC 4.2.2.5); xanthan lyase (EC 4.2.2.12); and chondroitin ABC lyase (EC 4.2.2.20).

Substrate specificities

PL8s are active on a variety of uronic acid-containing polysaccharides including hyaluronan [4)-β-D-Glucuronate-1,3-β -D-N-Acetyl-Glucosamine(1]_n, chondroitin AC [4)-β-D-Glucuronate-1,3-β-D-N-Acetyl-GalactosamineΔ4,6S(1]_n, xanthan [4)-β-D-Glucuronate-1,4-β-D-Glucuronate (1]_n, and chondroitin ABC [chondroitin AC and chondroitin B (aka. dermatan sulfate: 4)-β-L-Iduronate2S-1,3-β-D-N-Acetyl-Galactosamine4S(1]_n.

Kinetics and Mechanism

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Catalytic Residues

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Three-dimensional structures

Structure by Activity: Hyaluronidase – “Streptococcus pneumoniae” R6 [1](PDB 1OJM). Chondroitin AC lyase – “Bacteroides stercoris” HJ-15 [2](PDB 1CB8). Xanthanase – “Bacillus” sp GL1 [3](PDB 1J0M). Chondroitin ABC lyase – “Proteus vulgaris” [4](PDB 1HN0).

Family Firsts

First stereochemistry determination

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First catalytic nucleophile identification

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First general acid/base residue identification

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First 3-D structure

Chondroitin AC lyase – “Bacteroides stercoris” HJ-15 [2](PDB 1CB8).

First Structure by Activity: Hyaluronidase – “Streptococcus pneumoniae” R6 [1](PDB 1OJM). Chondroitin AC lyase – “Bacteroides stercoris” HJ-15 [2](PDB 1CB8). Xanthanase – “Bacillus” sp GL1 [3](PDB 1J0M). Chondroitin ABC lyase – “Proteus vulgaris” [4](PDB 1HN0).

References

<biblio>

1. Rapport1951 pmid=14917676
2. Li2000 pmid=10716923

1. Fethiere1999 pmid=10329169
2. Sato1994 pmid=7512814
3. Huang2003 pmid=12706721

1. Hashimoto1998 pmid=9758797
2. Hashimoto2003 pmid=16348550