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User:Takayuki Ohnuma
Takayuki Ohnuma is an assistant professor at Department of Advanced Bioscience, Faculty of Agriculture, Kinki University located in Nara, Japan.
He received PhD degree in 2002 from Graduate School of Agriculture in Kyushu University. His research program currently focuses on the structures and functions of plant chitinases (GH18 and GH19), including their CBMs (CBM18 and CBM50), which are involved in plant self-defense against fungal pathogens [1-11]. In 2013, He and his colabolater published [2] the structure of a complex of rye family 19 chitinase with two molecules of (GlcNAc)4. This is the first report on the complete subsite mapping of GH19 chitinase.
References
- Ohnuma T, Umemoto N, Nagata T, Shinya S, Numata T, Taira T, Fukamizo T. Crystal structure of a "loopless" GH19 chitinase in complex with chitin tetrasaccharide spanning the catalytic center. Biochim Biophys Acta, 1844, 793-802 (2014)
- Ohnuma T, Umemoto N, Kondo K, Numata T, Fukamizo T. Complete subsite mapping of a "loopful" GH19 chitinase from rye seeds based on its crystal structure. FEBS Lett, 587, 2691-7 (2013)
- Ohnuma T, Taira T, Fukamizo T. Antifungal activity of recombinant class V chitinases from Nicotiana tabacum and Arabidopsis thaliana. J Appl Glycosci, 59, 1, 47-50 (2012)
- Umemoto N, Ohnuma T, Mizuhara M, Sato H, Skriver K, Fukamizo T. Introduction of a tryptophan side chain into subsite +1 enhances transglycosylation activity of a GH-18 chitinase from Arabidopsis thaliana, AtChiC. Glycobiology, 23, 81-90 (2013)
- Ohnuma T, Numata T, Osawa T, Inanaga H, Okazaki Y, Shinya S, Kondo K, Fukuda T, Fukamizo T. Crystal structure and chitin oligosaccharide-binding mode of a 'loopful' family GH19 chitinase from rye, Secale cereale, seeds. FEBS J, 19, 3639-3651 (2012)
- Umemoto N, Ohnuma T, Urpilainen H, Yamamoto T, Numata T, Fukamizo T. Role of tryptophan residues in a class V chitinase from Nicotiana tabacum. Biosci Biotechnol Biochem, 76,778-784 (2012)
- Ohnuma T, Fukuda T, Dozen S, Honda Y, Kitaoka M, Fukamizo T. A glycosynthase derived from an inverting GH19 chitinase from the moss Bryum coronatum. Biochem J, 444, 437-443 (2012)
- Shinya S, Ohnuma T, Kawamura S, Torikata T, Nishimura S, Katoh E, Fukamizo T. Interaction of a goose-type lysozyme with chitin oligosaccharides as determined by NMR spectroscopy. J Biochem, 150, 569-577 (2011)
- Ohnuma T, Sørlie M, Fukuda T, Kawamoto N, Taira T, Fukamizo T. Chitin oligosaccharide binding to a family GH19 chitinase from the moss Bryum coronatum. FEBS J, 278, 3991-4001 (2011)
- Ohnuma T, Numata T, Osawa T, Mizuhara M, Lampela O, Juffer AH, Skriver K, Fukamizo T. A class V chitinase from Arabidopsis thaliana: gene responses, enzymatic properties, and crystallographic analysis. Planta, 234, 123-137 (2011)
- Ohnuma T, Numata T, Osawa T, Mizuhara M, Vårum KM, Fukamizo T. Crystal structure and mode of action of a class V chitinase from Nicotiana tabacum. Plant Mol Biol, 75, 291-304 (2011)