CAZypedia celebrates the life of Senior Curator Emeritus Harry Gilbert, a true giant in the field, who passed away in September 2025.
CAZypedia needs your help!
We have many unassigned pages in need of Authors and Responsible Curators. See a page that's out-of-date and just needs a touch-up? - You are also welcome to become a CAZypedian. Here's how.
Scientists at all career stages, including students, are welcome to contribute.
Learn more about CAZypedia's misson here and in this article. Totally new to the CAZy classification? Read this first.
Difference between revisions of "Glycoside Hydrolase Family 50"
| Line 1: | Line 1: | ||
<!-- RESPONSIBLE CURATORS: Please replace the {{UnderConstruction}} tag below with {{CuratorApproved}} when the page is ready for wider public consumption --> | <!-- RESPONSIBLE CURATORS: Please replace the {{UnderConstruction}} tag below with {{CuratorApproved}} when the page is ready for wider public consumption --> | ||
{{UnderConstruction}} | {{UnderConstruction}} | ||
| − | * [[Author]]: | + | * [[Author]]: ^^^Mirjam Czjzek^^^ |
* [[Responsible Curator]]: ^^^Mirjam Czjzek^^^ | * [[Responsible Curator]]: ^^^Mirjam Czjzek^^^ | ||
---- | ---- | ||
| Line 33: | Line 33: | ||
== Kinetics and Mechanism == | == Kinetics and Mechanism == | ||
| − | Actually, a potential retaining mechanism of this glycoside hydrolase | + | Actually, a potential retaining mechanism of this glycoside hydrolase family can only be inferred from analogy to clan GH-A enzymes {{CAZyDBlink}}GH50.html. No mechanistic or kintetic analysis demonstrating the stereochemical outcome of the reaction have been reported for this family to date. |
| Line 41: | Line 41: | ||
== Three-dimensional structures == | == Three-dimensional structures == | ||
| − | Unknown; from analogy to clan GH-A enzymes it can be inferred that the 3D structure will be based on a (β/ | + | Unknown; from analogy to clan GH-A enzymes it can be inferred that the 3D structure will be based on a (β/α)<sub>8</sub> barrel fold. |
Revision as of 06:18, 30 June 2010
This page is currently under construction. This means that the Responsible Curator has deemed that the page's content is not quite up to CAZypedia's standards for full public consumption. All information should be considered to be under revision and may be subject to major changes.
- Author: ^^^Mirjam Czjzek^^^
- Responsible Curator: ^^^Mirjam Czjzek^^^
| Glycoside Hydrolase Family GH50 | |
| Clan | GH-A |
| Mechanism | probably retaining |
| Active site residues | inferred from clan GH-A as two Glu |
| CAZy DB link | |
| https://www.cazy.org/GH50.html | |
Substrate specificities
To date, all characterized glycoside hydrolases of family 50 are β-agarases (EC 3.2.1.81) that cleave β-1,4 glycosidic bonds of agarose, releasing neoagaro-biose -tetraose and -hexaose [1, 2, 3, 4]. Three enzymes, Aga50A and Aga50D from Saccharophagus degradans and Aga50B from Vibrio sp. have been reported to be pure exo-β-agarases [5].
Kinetics and Mechanism
Actually, a potential retaining mechanism of this glycoside hydrolase family can only be inferred from analogy to clan GH-A enzymes https://www.cazy.org/GH50.html. No mechanistic or kintetic analysis demonstrating the stereochemical outcome of the reaction have been reported for this family to date.
Catalytic Residues
Unkown
Three-dimensional structures
Unknown; from analogy to clan GH-A enzymes it can be inferred that the 3D structure will be based on a (β/α)8 barrel fold.
Family Firsts
- Identification of first family member
- The family was created in Cazy based on the work of Sugano et al. [1].
- First stereochemistry determination
- not determined yet.
- First catalytic nucleophile identification
- not determined yet.
- First general acid/base residue identification
- not determined yet.
- First 3-D structure
- not determined yet.
References
Error fetching PMID 8193156:
Error fetching PMID 15307821:
Error fetching PMID 17028783:
Error fetching PMID 19802606:
- Error fetching PMID 8517750:
- Error fetching PMID 8193156:
- Error fetching PMID 15307821:
- Error fetching PMID 17028783:
- Error fetching PMID 19802606: