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Difference between revisions of "Glycoside Hydrolase Family 32"
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== Substrate specificities == | == Substrate specificities == | ||
| − | Family GH32 contains one of the earliest described enzyme activities, namely that of 'inverting' sucrose | + | Family GH32 contains one of the earliest described enzyme activities, namely that of 'inverting' sucrose, from which is derived the name of 'invertase'(EC 3.2.1.26), discovered in the second half of the 19th century <cite>1</cite>. Besides the 'historical' invertases, this family also contains enzymes active on fructose containing polysaccharides such as inulinases (EC 3.2.1.7) and exo-inulinases (EC 3.2.1.80), levanases (EC 3.2.1.65) and -2,6-fructan 6-levanbiohydrolases(EC 3.2.1.64), fructan -(2,1)-fructosidase/1-exohydrolase (EC 3.2.1.153) or fructan -(2,6)-fructosidase/6-exohydrolases (EC 3.2.1.154), as well as enzymes displaying transglycosylating activites such as sucrose:sucrose 1-fructosyltransferases (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). |
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== Kinetics and Mechanism == | == Kinetics and Mechanism == | ||
Revision as of 08:16, 20 July 2009
| Glycoside Hydrolase Family GH32 | |
| Clan | GH-J |
| Mechanism | retaining |
| Active site residues | known |
| CAZy DB link | |
| http://www.cazy.org/fam/GH32.html | |
Substrate specificities
Family GH32 contains one of the earliest described enzyme activities, namely that of 'inverting' sucrose, from which is derived the name of 'invertase'(EC 3.2.1.26), discovered in the second half of the 19th century [1]. Besides the 'historical' invertases, this family also contains enzymes active on fructose containing polysaccharides such as inulinases (EC 3.2.1.7) and exo-inulinases (EC 3.2.1.80), levanases (EC 3.2.1.65) and -2,6-fructan 6-levanbiohydrolases(EC 3.2.1.64), fructan -(2,1)-fructosidase/1-exohydrolase (EC 3.2.1.153) or fructan -(2,6)-fructosidase/6-exohydrolases (EC 3.2.1.154), as well as enzymes displaying transglycosylating activites such as sucrose:sucrose 1-fructosyltransferases (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-).
Kinetics and Mechanism
Catalytic Residues
Three-dimensional structures
Family Firsts
- First sterochemistry determination
- Cite some reference here, with a short explanation [1].
- First catalytic nucleophile identification
- First general acid/base residue identification
- First 3-D structure
References
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O'Sullivan, C., and Tompson, F. W. (1890) J. Chem. Soc. 57, 854-870