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Difference between revisions of "Glycoside Hydrolase Family 50"
| Line 29: | Line 29: | ||
== Substrate specificities == | == Substrate specificities == | ||
| − | To date, all characterized [[glycoside hydrolases]] of family 50 are &beta-agarases (EC [{{EClink}}3.2.1.81 3.2.1.81]) that cleave β-1,4 glycosidic bonds of agarose, releasing neoagaro-biose, -tetraose or -hexaose. Three enzymes, Aga50A and Aga50D from Saccharophagus degradans and Aga50B from Vibrio sp. have been reported to be pure exo-&beta-agarases <cite>REF1</cite> | + | To date, all characterized [[glycoside hydrolases]] of family 50 are β-agarases (EC [{{EClink}}3.2.1.81 3.2.1.81]) that cleave β-1,4 glycosidic bonds of agarose, releasing neoagaro-biose, -tetraose or -hexaose. Three enzymes, Aga50A and Aga50D from Saccharophagus degradans and Aga50B from Vibrio sp. have been reported to be pure exo-β-agarases <cite>REF1</cite> |
| Line 45: | Line 45: | ||
== Family Firsts == | == Family Firsts == | ||
| − | ;First stereochemistry determination: Cite some reference here, with a ''short'' (1-2 sentence) explanation | + | ;First stereochemistry determination: Cite some reference here, with a ''short'' (1-2 sentence) explanation . |
| − | ;First catalytic nucleophile identification: Cite some reference here, with a ''short'' (1-2 sentence) explanation | + | ;First catalytic nucleophile identification: Cite some reference here, with a ''short'' (1-2 sentence) explanation. |
| − | ;First general acid/base residue identification: Cite some reference here, with a ''short'' (1-2 sentence) explanation | + | ;First general acid/base residue identification: Cite some reference here, with a ''short'' (1-2 sentence) explanation. |
| − | ;First 3-D structure: Cite some reference here, with a ''short'' (1-2 sentence) explanation | + | ;First 3-D structure: Cite some reference here, with a ''short'' (1-2 sentence) explanation. |
== References == | == References == | ||
<biblio> | <biblio> | ||
#REF1 pmid=8193156 | #REF1 pmid=8193156 | ||
| − | + | ||
| − | |||
| − | |||
</biblio> | </biblio> | ||
[[Category:Glycoside Hydrolase Families|GH050]] | [[Category:Glycoside Hydrolase Families|GH050]] | ||
Revision as of 11:59, 29 May 2010
This page is currently under construction. This means that the Responsible Curator has deemed that the page's content is not quite up to CAZypedia's standards for full public consumption. All information should be considered to be under revision and may be subject to major changes.
- Author:
- Responsible Curator: ^^^Mirjam Czjzek^^^
| Glycoside Hydrolase Family GH50 | |
| Clan | GH-A |
| Mechanism | probably retaining |
| Active site residues | inferred from clan GH-A as two Glu |
| CAZy DB link | |
| https://www.cazy.org/GH50.html | |
Substrate specificities
To date, all characterized glycoside hydrolases of family 50 are β-agarases (EC 3.2.1.81) that cleave β-1,4 glycosidic bonds of agarose, releasing neoagaro-biose, -tetraose or -hexaose. Three enzymes, Aga50A and Aga50D from Saccharophagus degradans and Aga50B from Vibrio sp. have been reported to be pure exo-β-agarases [1]
Kinetics and Mechanism
Content is to be added here.
Catalytic Residues
Content is to be added here.
Three-dimensional structures
Content is to be added here.
Family Firsts
- First stereochemistry determination
- Cite some reference here, with a short (1-2 sentence) explanation .
- First catalytic nucleophile identification
- Cite some reference here, with a short (1-2 sentence) explanation.
- First general acid/base residue identification
- Cite some reference here, with a short (1-2 sentence) explanation.
- First 3-D structure
- Cite some reference here, with a short (1-2 sentence) explanation.